电离辐射通过转化生长因子-β-内源性的上皮-间质转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

参考资料 ：探讨放射是不是可通过产物糖细胞内-β（TGF-β）-抑制的视网膜-间质转换成 (EMT)来推动肿瘤细胞的蹂躏迁到。可用据统计2Gy(60)Coγ线或反射来源于人类器官的6种肿瘤细胞，记录与EMT相关的推移，这包括分别借助于显微镜技术，细胞内质其内方法有，免疫荧光技术，水痘试验和Transwell小室试验来观察并探测细胞组织基本上，EMT记号，蹂躏迁到灵活性等。采用核糖体联免疫吸附法探测这些肿瘤细胞中都TGF-β细胞内技术水平，借助于特别抑制剂SB431542来评估TGF-β波形通路在放射EMT中都的作用。经过据统计为2Gy反射的肿瘤细胞中都假定间叶细胞的表达出来，与假反射组相比其视网膜记号下降，间叶细胞记号增加，同时其蹂躏移转到灵活性增强，TGF-β细胞内技术水平也提高。促使发现由A549放射诱导的EMT可通过对TGF-β波形抑制发生翻盘。这些表明TGF-β抑制的EMT在放射诱导增强肿瘤细胞蹂躏移转到灵活性中都起着关键作用。

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